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Affinity of Cefmenoxime for Beta-Lactamases: An Analysis

  • Author Footnotes
    3 Museum National d'Histoire Naturelle-C.N.R.S. (UA 401), Paris, France
    Roger Labia
    Correspondence
    Requests for reprints should be addressed to Dr. R. Labia, Museum National d'Histoire Naturelle, 63, rue Buffon, 75005 Paris, France.
    Footnotes
    3 Museum National d'Histoire Naturelle-C.N.R.S. (UA 401), Paris, France
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    Paris, France
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  • Author Footnotes
    3 Museum National d'Histoire Naturelle-C.N.R.S. (UA 401), Paris, France
    Annick Morand
    Footnotes
    3 Museum National d'Histoire Naturelle-C.N.R.S. (UA 401), Paris, France
    Affiliations
    Paris, France
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  • Author Footnotes
    3 Museum National d'Histoire Naturelle-C.N.R.S. (UA 401), Paris, France
    Catherine Verchere-Beaur
    Footnotes
    3 Museum National d'Histoire Naturelle-C.N.R.S. (UA 401), Paris, France
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    Paris, France
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  • Author Footnotes
    4 Laboratoire de Bactériologie, C.H.G., Argenteuil, France.
    André Bryskier
    Footnotes
    4 Laboratoire de Bactériologie, C.H.G., Argenteuil, France.
    Affiliations
    Argenteuil, France
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  • Author Footnotes
    3 Museum National d'Histoire Naturelle-C.N.R.S. (UA 401), Paris, France
    4 Laboratoire de Bactériologie, C.H.G., Argenteuil, France.
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      The interactions of cefmenoxime with beta-lactamases in comparison with cefotaxime, moxalactam, cefoperazone, and ceftazidime have been determined. On-line computerized microacidimetry allowed determination of the affinity of these compounds with the enzymes, which was characterized by Km values. The beta-lactamaser that were used were two cephalosporinases and one penicillinase. Within these data, the cephalosporins could be classified into three groups: (1) those with high affinity for the cephalosporinases and very poor affinity for the penicillinase (cefmenoxime, cefotaxime, and moxalactam); (2) those with moderate affinity for the cephalosporinases and very poor affinity for the penicillinase (ceftazidime); (3) those with poor affinity for all enzymes (cefoperazone). In the case of the penicillinase (TEM-1), only cefoperazone was subject to some hydrolysis.
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