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Plasma fibronectin as a marker for cancer and other diseases

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      Abstract

      The blood concentration of the high-molecular-weight glycoprotein fibronectin showed great promise as a marker for cancer and certain other disease states. However, it now appears that plasma fibronectin levels are influenced by many factors (e.g., age, sex, nutritional state, trauma, shock, inflammation, certain drugs), sometimes giving conflicting responses. Furthermore, unrecognized pitfalls exist in fibronectin determinations with respect to blood sampling methods, plasma preparation, plasma storage, and re-use and assay methods. It is concluded that the plasma fibronectin level, as frequently reported, has little value as a marker for cancer and other disease states.
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      References

        • Yamada KM
        • Olden K
        Fibronectins: adhesive glycoproteins of cell surface and blood.
        Nature. 1978; 275: 179-184
        • Pearlstein E
        • Gold LI
        • Garcia-Pardo A
        Fibronectin: a review of its structure and biological activity.
        Mol Cell Biochem. 1980; 29: 103-128
        • Ruoslahti E
        • Hayman EG
        • Engvall E
        • Cothran WC
        • Butler WT
        Alignment of biologically active domains in the fibronectin molecule.
        J Blol Chem. 1981; 256: 7277-7281
        • Sekiguchi K
        • Hakomori SI
        Functional domain structure of fibronectin.
        in: 2nd ed. Proc Natl Acad Sci USA. 77. 1980: 2661-2665
        • Stenman S
        • Vahen A
        Distribution of a major connective tissue protein, fibronectin, in normal human tissues.
        J Exp Med. 1978; 147: 1054-1064
        • Vaheri A
        • Alitalo K
        • Hedman K
        • Keski-Oja J
        • Kurkinen M
        • Wartiovaara J
        Fibronectin and the pericellular matrix of normal and transformed adherent cells.
        Ann NY Acad Sci. 1978; 312: 343-353
        • Yamada KM
        • Kennedy DW
        Fibroblast cellular and plasma fibronectins are similar but not identical.
        J Cell Biol. 1979; 80: 492-498
        • Grinnell F
        • Feld M
        • Minter D
        Fibroblast adhesion to fibrinogen and fibrin substrata: requirement for cold-insoluble globulin (plasma fibronectin).
        Cell. 1980; 19: 517-525
        • Hynes RO
        • Ali IU
        • Destree AT
        • et al.
        A large glycoprotein lost from the surfaces of transformed cells.
        Ann NY Acad Sci. 1978; 312: 317-342
        • Hynes RO
        • Destree AT
        • Perkins ME
        • Wagner DD
        Cell surface fibronectin and oncogenic transformation.
        J Supramol Struct. 1979; 11: 95-104
        • Vaheri A
        • Mosher DF
        High molecular weight, cell surface-associated glycoprotein (fibronectin) lost in malignant transformation.
        Biochim Biophys Acta. 1978; 516: 1-25
        • Blumberg PM
        • Drieger PE
        • Rossow PW
        Effect of a phorbol ester on a transformation-sensitive surface protein of chick fibroblasts.
        Nature. 1976; 264: 446-447
        • Bolmer SD
        • Wolf G
        Retinoids and phorbol esters alter release of fibronectin from enucleated cells.
        in: 2nd ed. Proc Natl Acad Sci USA. 79. 1982: 6541-6545
        • Keski-Oja J
        • Shoyab M
        • De Larco JE
        • Todaro GJ
        Rapid release of fibronectin from human lung fibroblasts by biologically active phorbol esters.
        Int J Cancer. 1979; 24: 218-224
        • Mosesson MW
        • Umfleet RA
        The cold insoluble globulin of human plasma. I. Purification, primary characterization, and relationship to fibrinogen and other cold insoluble fraction components.
        J Biol Chem. 1970; 245: 5728-5736
        • Balian G
        • Crouch E
        • Click EM
        • Carter WG
        • Bornstein P
        Comparison of the structures of human fibronectin and plasma cold-insoluble globulin.
        J Supramol Struct. 1979; 12: 505-516
        • Kuusela P
        • Vaheri A
        • Palo J
        • Ruoslahti E
        Demonstration of fibronectin in human cerebrospinal fluid.
        J Lab Clin Med. 1978; 92: 595-601
        • Engvall E
        • Ruoslahti E
        Binding of soluble form of fibroblast surface protein, fibronectin, to collagen.
        Int J Cancer. 1977; 20: 1-5
        • Stathakis NE
        • Mosesson MW
        Interactions among heparin, cold-insoluble globulin, and fibrinogen in formation of the heparin-precipitable fraction of plasma.
        J Clin Invest. 1977; 60: 855-865
        • Yamada KM
        • Kennedy DW
        • Kimata K
        • Pratt RM
        Characterization of fibronectin interactions with glycosaminoglycans and identification of active proteolytic fragments.
        J Biol Chem. 1980; 255: 6055-6063
        • Stemberger AW
        • Hormann H
        Determination of soluble fibrin monomer complexes by adsorption on immobilized fibrinogen.
        Thromb Haemost. 1978; 39: 574-581
        • Ruoslahti E
        • Vaheri A
        Interaction of soluble fibroblast surface antigen with fibrinogen and fibrin.
        J Exp Med. 1975; 141: 497-501
        • Hynes RO
        • Destree AT
        Relationships between fibronectin (LETS protein) and actin.
        Cell. 1978; 15: 875-886
        • Keski-Oja J
        • Yamada KM
        Isolation of an actin-binding fragment of fibronectin.
        Biochem J. 1981; 193: 615-620
        • Menzel EJ
        • Smolen JS
        • Liotta L
        • Reid KBM
        Interaction of fibronectin with C1q and its collagen-like fragment (CLF).
        FEBS Lett. 1981; 129: 188-192
        • Kuusela P
        Fibronectin binds to Staphylococcus aureus.
        Nature. 1978; 276: 718-720
        • Saba TM
        • Jaffe E
        Plasma fibronectin (opsonic glycoprotein): its synthesis by vascular endothelial cells and role in cardiopulmonary integrity after trauma as related to reticuloendothelial function.
        Am J Med. 1980; 68: 577-594
        • Vuento M
        • Korkolainen M
        • Stenman UH
        Fibronectin binds to charge-modified proteins.
        Adv Exp Med Biol. 1982; 155: 623-628
        • Mosher DF
        Cross-linking of cold-insoluble globulin by fibrin-stabilizing factor.
        J Biol Chem. 1975; 250: 6614-6621
        • Rouslahti E
        • Hayman EG
        • Pierschbacher M
        • Engvall E
        Fibronactin: purification, immunochemical properties, and biological activities.
        Methods Enzymol. 1982; 82: 803-831
        • Bowen M
        • Muller T
        Influence of sample preparation on estimates of blood fibronectin concentration.
        J Clin Pathol. 1983; 36: 233-235
        • Eriksen HO
        • Clemmensen I
        • Hansen MS
        • Ibsen KK
        Plasma fibronectin concentration in normal subjects.
        Scand J Clin Lab Invest. 1982; 42: 291-295
        • Richter H
        • Seidl M
        • Hormann H
        Location of heparin-binding sites of fibronectin: detection of a hitherto unrecognized transamidase sensitive site.
        Hoppe Seylers Z Physiol Chem. 1981; 362: 399-408
        • Mosesson MW
        • Amrani DL
        The structure and biologic activities of plasma fibronectin.
        Blood. 1980; 56: 145-158
        • Fyrand O
        • Solum NO
        Studies on cold insoluble globulin in dermatological patients. I. Immunochemical quantitation in citrated plasma from patients with increased amounts of heparin precipitable fraction (HPF).
        Thromb Res. 1976; 9: 447-455
        • Mosher DF
        • Williams EM
        Fibronectin concentration is decreased in plasma of severely ill patients with disseminated intravascular coagulation.
        J Lab Clin Med. 1978; 91: 729-735
        • Labat-Robert J
        • Potazman JP
        • Derouette JC
        • Robert L
        Age-dependent increase of human plasma fibronectin.
        Cell Biol Int Rep. 1981; 5: 969-973
        • Choate JJ
        • Mosher DF
        Fibronectin concentration in plasma of patients with breast cancer, colon cancer, and acute leukemia.
        Cancer. 1983; 51: 1142-1147
        • Klingemann HG
        • Kosukavak M
        • Hofeier H
        • Havemann K
        Fibronectin and factor VIII-related antigen in acute leukaemia.
        Hoppe Seylers Z Physiol Chem. 1983; 364: 269-277
        • Todd HD
        • Coffee MS
        • Waalkes TP
        • Abeloff MD
        • Parsons RG
        Serum levels of fibronectin and a fibronectin-like DNA-binding protein in patients with various diseases.
        JNCI. 1980; 65: 901-904
        • Saba TM
        • Albert WH
        • Blumenstock FA
        • Evanega G
        • Staehler F
        • Cho E
        Evaluation of a rapid immunoturbidimetric assay for opsonic fibronectin in surgical and trauma patients.
        J Lab Clin Med. 1981; 98: 482-491
        • Gonzalez-Calvin J
        • Scully MF
        • Sanger Y
        • et al.
        Fibronectin in fulminant hepatic failure.
        Br Med J [Clin Res]. 1982; 285: 1231-1232
        • Matsuda M
        • Yamanaka T
        • Matsuda A
        Distribution of fibronectin in plasma and liver in liver diseases.
        Clin Chim Acta. 1982; 118: 191-199
        • Pearlstein E
        • Baez L
        A solid-phase radioimmunoassay for the determination of fibronectin levels in plasma.
        Anal Biochem. 1981; 116: 292-297
        • Alexander CM
        • Lum SM
        • Rhodes J
        • Boarman C
        • Nicoloff JT
        • Kumar D
        Rapid increase in both plasma fibronectin and serum triiodothyronine associated with treatment of diabetic ketoacidosis.
        J Clin Endocrinol Metab. 1983; 56: 279-282
        • Todd-Kulikowski HD
        • Parsons RG
        A stable, sensitive assay for human fibronectin.
        J Immunol Methods. 1981; 44: 333-341
        • Plow EF
        • Birdwell C
        • Ginsberg MH
        Identification and quantitation of platelet-associated fibronectin antigen.
        J Clin Invest. 1979; 63: 540-543
        • Parenti D
        • Carsons SE
        • Lavietes BB
        • Diamond HS
        • Steinman CR
        Fibronectin, a DNA binding protein, is elevated in systemic lupus erythematosus.
        Arthritis Rheum. 1982; 25 (abstr 234) (suppl): S41
        • Scon RL
        • Sohmer PR
        • MacDonald MBG
        The effect of starvation and repletion on plasma FN in man.
        JAMA. 1982; 248: 2025-2027
        • Vaheri A
        • Vartio T
        • Stenman S
        • Saksela O
        • Hedman K
        • Alitalo k
        Fibronectin and proteinases in tumor invasion.
        in: Strauli P Barrett AJ Baici A Proteinases and tumor invasion. Raven Press, New York1980: 49-57
        • Chen AB
        • Amrani DL
        • Mosesson MW
        Heterogeneity of the cold-insoluble globulin of human plasma (C1q), a circulating cell surface protein.
        Biochim Biophys Acta. 1977; 493: 310-322
        • Clemmensen I
        • Andersen RB
        Different molecular forms of fibronectin in rheumatoid synovial fluid.
        Arthritis Rheum. 1982; 25: 25-31
        • Saba TM
        • Antikatzides TG
        Humoral mediated macrophage response during tumour growth.
        Br J Cancer. 1975; 32: 471-482
        • Labat-Robert J
        • Birembaut P
        • Adnet JJ
        • Mercantini F
        • Robert L
        Loss of fibronectin in human breast cancer.
        Cell Biol Int Rep. 1980; 4: 609-616
        • Stenman S
        • Vaheri A
        Fibronectin in human solid tumors.
        Int J Cancer. 1981; 27: 427-435
        • Smith HS
        • Riggs JL
        • Mosesson MW
        Production of fibronectin (LETS protein) by human epithelial cells in culture.
        Cancer Res. 1979; 39: 4138-4145
        • Taylor-Papadimitriou J
        • Burchell J
        • Hurst J
        Production of fibronectin by normal and malignant human mammary epithelial cells.
        Cancer Res. 1981; 41: 2491-2500
        • Stampfer MR
        • Vlodavsky I
        • Smith HS
        • Ford R
        • Becker FF
        • Riggs J
        Fibronectin production by human mammary cells.
        JNCI. 1981; 67: 253-261
        • Isemura M
        • Yosizawa Z
        • Kojima N
        • Ishida F
        Fibronectin in human hepatocellular carcinoma.
        Tohoku J Exp Med. 1982; 138: 441-442
        • Forkman B
        • Ganrot PO
        • Gennser G
        • Rannevik G
        Plasma protein pattern in recurrent cholestasis of pregnancy.
        Scand J Clin Lab Invest. 1972; 29 (suppl): 89-96
        • Saba TM
        • Blumenstock FA
        • Weber P
        • Kaplan JE
        Physiologic role of cold-insoluble globulin in systemic host defense: implications of its characterization as the opsonic alpha 2-surface-binding glycoprotein.
        Ann NY Acad Sci. 1978; 312: 43-55
        • Blumenstock FA
        • Weber P
        • Saba TM
        Purification of alpha-2-opsonic protein from human serum and its measurement by immunoassay.
        J Reticuloendothel Soc. 1978; 23: 119-134
        • Blumenstock FA
        • Saba TM
        Fibronectin: a mediator of phagocytosis and its potential in treating septic shock.
        Adv Exp Med Biol. 1982; 155: 613-621
        • Scovill WA
        • Saba TM
        • Blumenstock FA
        • Bernard H
        • Powers SR
        Opsonic α2 surface binding glycoprotein therapy during sepsis.
        Ann Surg. 1978; 188: 521-529
        • Bruhn HD
        • Heimburger N
        Factor-VIII-related antigen and cold insoluble globulin in leukemias and carcinomas.
        Haemostasis. 1976; 5: 189-192
        • Goldman AS
        • Rudloff HB
        • McNamee R
        • Loose LD
        • Diluzio NR
        Deficiency of plasma humoral recognition factor activity following burn injury.
        J Reticuloendothel Soc. 1973; 15: 193-197
        • Zardi L
        • Cecconi C
        • Barbieri O
        • Carnemolla B
        • Picea M
        • Santi L
        Concentration of fibronectin in plasma of tumor-bearing mice and synthesis by Ehrlich ascites tumor cells.
        Cancer Res. 1979; 39: 3374-3379
        • Mosher DF
        Changes in plasma cold-insoluble globulin concentration during experimental Rocky Mountain spotted fever infection in rhesus monkeys.
        Thromb Res. 1976; 9: 37-45