A controlled study of the efficacy of granulocyte transfusions in patients with neutropenia

      This paper is only available as a PDF. To read, Please Download here.


      A randomized clinical trial to determine the efficacy of granulocyte transfusions in neutropenic patients with infection was conducted. Criteria for patient selection included a proved infection, a granulocyte count of less than 300/mm3, availability of a suitable donor and failure to respond to at least 72 hours of appropriate antibiotic therapy. Thirty patients were assigned at random to receive either granulocyte transfusions or to serve as a control group. Antibiotic therapy was continued in both groups. Responses were judged by the degree of diminution of infectious episodes and survival. The results showed that 11 of 13 control patients failed to respond during the period of observation, whereas 10 of 17 patients given transfusions responded. The results were statistically significantly different (p < 0.05). The median survival was 22.5 days in the group given transfusions (group 2) and 7.7 in the control group (group 1) (p < 0.01). The granulocyte transfusions were most effective in patients with hypocellular marrows who failed to recover during the period of observation. These results indicate that granulocyte transfusions are effective in the short-term control of infections in neutropenic patients.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to The American Journal of Medicine
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Levine AS
        • Siegel SE
        • Schreiber AD
        • et al.
        Protected environments and prophylactic antibiotics.
        N Engl J Med. 1973; 288: 477
        • Levine AS
        • Schimpff SC
        • Graw RG
        • et al.
        Hematologic malignancies and other marrow failure states. Progress in the management of complicating infections.
        in: Semin Hematol. 11. 1974: 141
        • Schimpff S
        • Satterlee W
        • Young VM
        • et al.
        Therapy with carbenicillin and gentamicin for febrile cancer patients.
        N Engl J Med. 1971; 284: 1061
        • Klastersky J
        • Cappel R
        • Daneau D
        Therapy with carbenicillin and gentamicin for patients with cancer and severe infections caused by gram-negative rods.
        Cancer. 1973; 31: 331
        • Rodriguez V
        • Burgess M
        • Bodey GP
        Management of fever of unknown origin in patients with neoplasms and neutropenia.
        Cancer. 1973; 32: 1007
        • Tattersall MHN
        • Spiers ASD
        • Darrell JH
        Initial therapy with combination of five antibiotics in febrile patients with leukemia and neutropenia.
        Lancet. 1972; 1: 162
        • Bloomfield CD
        • Kennedy BJ
        Cephalothin, carbenicillin, and gentamicin combination therapy for febrile patients with acute non-lymphocytic leukemia.
        Cancer. 1974; 34: 431
        • Bodey GP
        • Hart J
        • Freireich EJ
        • et al.
        Studies of a patient isolator unit and prophylactic antibiotics in cancer chemotherapy.
        Cancer. 1968; 22: 1018
        • Yates JW
        • Holland JF
        A controlled study of isolation and endogenous microbial suppression in acute myelocytic leukemia patients.
        Cancer. 1973; 32: 1490
        • Schimpff SC
        • Greene WH
        • Young BM
        • et al.
        Infection prevention in acute non-lymphocytic leukemia (laminar air flow room reverse isolation with oral, non-absorbable antibiotic prophylaxis).
        Ann Intern Med. 1975; 83: 351
        • Bodey FP
        • Rodriguez V
        Infections in cancer patients on a protected environment-prophylactic antibiotic program.
        Am J Med. 1975; 59: 497
        • Graw RG
        • Herzig G
        • Perry S
        Normal granulocyte transfusion therapy.
        N Engl J Med. 1972; 287: 367
        • McCredie KV
        • Freireich EJ
        • Hester JP
        Leukocyte transfusions therapy for patients with host-defense failure.
        Transplant Proc. 1973; 5: 1285
        • Lowenthal RM
        • Goldman JM
        • Buskard NA
        • et al.
        Granulocyte transfusions in treatment of infections in patients with acute leukemia and aplastic anemia.
        Lancet. 1975; 1: 353
        • Schiffer CA
        • Buchholz DH
        • Aisner J
        • et al.
        Clinical experience with transfusion of granulocytes obtained by continuous flow filtration leukapheresis.
        Am J Med. 1975; 58: 373
        • Higby DJ
        • Yates JW
        • Henderson ES
        • et al.
        Filtration leukapheresis for granulocyte transfusions therapy (clinical and laboratory studies).
        N Engl J Med. 1975; 292: 761
        • Bodey GP
        • Buckley M
        • Sathe YS
        • et al.
        Quantitative relationships between circulating leucocytes and infection in patients with acute leukemia.
        Ann Intern Med. 1966; 64: 328
        • Hughes WT
        • Smith DR
        Infection during induction of remission in acute lymphocytic leukemia.
        Cancer. 1973; 31: 1008
        • Van Roode JJ
        • van Leeuwan A
        An agglutination technique for the demonstration of leucocyte isoantigens in man using leucocytes from EOTA blood. Histocompatibility Testing.
        in: Publication No. 1229. National Academy of Science-National Research Council, Washington, D.C1965: 153
        • Shoji M
        • Vogler WR
        Effects of hydrocortisone on the yield and bactericidal function of granulocytes collected by continuous-flow centrifugation.
        Blood. 1974; 44: 435
        • Gehan EA
        A generalized Wilcoxon test for comparing arbitrarily singly-censored samples.
        Biometrika. 1965; 52: 203
        • Herzig RH
        • Herzig GP
        • Graw Jr, RG
        • et al.
        Granulocyte transfusion therapy for gram-negative septicemia.
        N Engl J Med. 1977; 296: 701
        • Alavi JB
        • Root RK
        • Djerassi I
        • et al.
        Clinical trial of granulocyte transfusions for infection in acute leukemia.
        N Engl J Med. 1977; 296: 706
        • Djerassi I
        • Kim JS
        • Mitrakul C
        • et al.
        Filtration leukapheresis for separation and concentration of transfusable amounts of normal human granulocytes.
        J Med (Basel). 1970; I: 358
        • Harris MD
        • Djerassi I
        • Schwartz E
        • et al.
        Polymorphonuclear leukocytes prepared by continuous-flow filtration leukapheresis: variability and function.
        Blood. 1974; 44: 707
        • Herzig GP
        • Root RK
        • Graw RG
        Granulocyte collection by continuous-flow filtration leukapheresis.
        Blood. 1972; 39: 554