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RNA metabolism and protein synthesis in plasma cell leukemia

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      Abstract

      A patient with plasma cell leukemia was found to have Bence Jones proteinemia and Bence Jones proteinuria.
      Peripheral blood plasma cell suspensions incorporated C14-uridine into RNA and C14-leucine into protein, including Bence Jones protein. Approximately 81 per cent of the newly-formed RNA was unstable, and was destroyed with a half-life of about 42 minutes. This RNA may represent messenger RNA or unstable nuclear RNA. Inhibition of RNA synthesis by actinomycin D was followed by a marked and progressive decline in the rate of protein synthesis. Secretion of Bence Jones protein lagged 2 hours behind protein synthesis.
      These data demonstrate production of an immunoglobulin by leukemic plasma cells, selective secretion of Bence Jones protein and instability of messenger RNA's, including specific Bence Jones messengers. The leukemic plasma cells, like normal plasma cells, require continuing gene action (RNA synthesis) to support growth and differentiation.
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      References

        • Curtain C.C.
        • O'Dea J.F.
        Possible sites of macroglobulin synthesis: a study made with fluorescent antibody.
        Australasian Ann. Med. 1959; 2: 143
        • Dutcher T.F.
        • Fahey J.L.
        Immunocytochemical demonstration of intranuclear localization of 18 S gamma macroglobulin in macroglobulinemia of Waldenström.
        in: Proc. Soc. Exper. Biol. & Med.103. 1960: 452
        • Curtain C.C.
        Localization of 18 S gamma-macroglobulin in plasmacytoid cells of the bone marrow of a case of macroglobulinaemia.
        Australian J. Exper. Biol. & M. Sc. 1961; 39: 391
        • Solomon A.
        • Fahey J.L.
        • Malmgren R.A.
        Immunohistologic localization of gamma-1-macroglobulins, beta-2A-myeloma proteins, 6.6S gamma-myeloma proteins and Bence Jones proteins.
        Blood. 1963; 21: 403
        • Sonnet J.
        • Gillant C.
        • Sokal G.
        Démonstration de la synthése de paraprotéines sériques par des plasmocytes, grâce au glucose uniformément marqué par le C14.
        Rev. belge path. et méd. exper. 1958; 26: 313
        • Zucker-Franklin D.
        • Franklin E.C.
        • Cooper N.S.
        Production of macroglobulins in vitro and a study of their cellular origin.
        Blood. 1962; 20: 56
        • Cooperband S.R.
        • Keimowitz R.M.
        Protein synthesis by blood and marrow lymphocytes from a patient with Waldenström's macroglobulinemia.
        Arthritis & Rheumat. 1965; 8: 437
        • Fahey J.L.
        • Horbett A.P.
        Human gamma globulin fractionation on anion exchange cellulose columns.
        J. Biol. Chem. 1959; 234: 2645
        • Putnam F.W.
        • Easley C.W.
        • Lynn L.T.
        • Ritchie A.E.
        • Phelps R.A.
        Heat precipitation of Bence-Jones proteins. I. Optimum conditions.
        Arch. Biochem. 1959; 83: 115
      1. Mullinax, F. In preparation.

        • Solomon A.
        • Fahey J.L.
        Bence Jones proteinemia.
        Am. J. Med. 1964; 37: 206
        • Helmreich E.
        • Kern M.
        • Eisen H.N.
        The secretion of antibody by isolated lymph node cells.
        J. Biol. Chem. 1961; 236: 464
        • Harris H.
        Rapidly labelled ribonucleic acid in the cell nucleus.
        Nature. 1963; 198: 184
        • Lieberman I.
        • Adams R.
        • Ove P.
        Changes in the metabolism of ribonucleic acid preceding the synthesis of deoxyribonucleic acid in mammalian cells cultured from the animal.
        J Biol Chem. 1963; 238: 2141
        • Roberts W.K.
        Studies on ribonucleic acid synthesis in Ehrlich ascites cells. Extraction and properties of labeled ribonucleic acid.
        Biochim. et biophys. acta. 1965; 108: 474
        • Perry R.P.
        The cellular sites of synthesis of ribosomal and 4 S RNA.
        in: Proc. Nat. Acad. Sc.48. 1962: 2179
        • Penman S.
        • Scherrer K.
        • Becker Y.
        • Darnell J.E.
        Polyribosomes in normal and poliovirus-infected HeLa cells and their relationship to messenger-RNA.
        in: Proc. Nat. Acad. Sc.49. 1963: 654
      2. Mullinax, F. and Mullinax, G. L. Submitted for publication.

        • Davidson E.H.
        • Allfrey V.G.
        • Mirsky A.E.
        Gene expression in differentiated cells.
        in: Proc. Nat. Acad. Sc.49. 1963: 1
        • Smiley J.D.
        • Heard J.G.
        • Ziff M.
        Effect of actinomycin D on RNA synthesis and antibody formation in the anamnestic response in vitro.
        J. Exper. Med. 1964; 119: 881
        • Villa-Trevino S.
        • Farber E.
        • Staehlin T.
        • Wettstein F.O.
        • Noll H.
        Breakdown and reassembly of rat liver ergosomes after administration of ethionine or puromycin.
        J. Biol. Chem. 1964; 239: 3826
        • Scott R.B.
        • Bell E.
        Messenger RNA utilization during development of chick embryo lens.
        Science. 1965; 147: 405
        • Bloom S.
        • Goldberg B.
        • Green H.
        The lifetime of messenger RNA for collagen and cell protein synthesis in an established cell line.
        Biochem. & Biophys. Res. Commun. 1965; 19: 317
        • Marks P.A.
        • Burka E.R.
        • Schlessinger D.
        Protein synthesis in erythroid cells. I. Reticulocyte ribosomes active in stimulating amino acid incorporation.
        in: Proc. Nat. Acad. Sc.48. 1962: 2163
        • Seed R.W.
        • Goldberg I.H.
        Biosynthesis of thyroglobulin: relationship to RNA-template and precursor protein.
        in: Proc. Nat. Acad. Sc.50. 1963: 275
        • Sainte-Marie G.
        • Coons A.H.
        Studies on antibody production. X. Mode of formation of plasmocytes in cell transfer experiments.
        J. Exper. Med. 1964; 119: 743
        • Kern M.
        • Helmreich E.
        • Eisen H.N.
        The solubilization of microsomal antibody activity by the specific interaction between the crystallizable fraction of gamma-globulin and lymph-node microsomes.
        in: Proc. Nat. Acad. Sc.47. 1961: 767
        • Helmreich E.
        • Kern M.
        • Eisen H.N.
        Observations on the mechanism of secretion of γ-globulin by isolated lymph node cells.
        J. Biol. Chem. 1962; 237: 1925
        • Warshawsky H.
        • Leblond C.P.
        • Droz B.
        Synthesis and migration of proteins in the cells of the exocrine pancreas as revealed by specific activity determination from radioautographs.
        J. Cell Biol. 1963; 16: 1
        • Caro L.G.
        • Palade G.E.
        Protein synthesis, storage, and discharge in the pancreatic exocrine cell.
        J. Cell Biol. 1964; 20: 473
        • Hendler R.W.
        Importance of membranes in protein biosynthesis.
        Nature. 1965; 207: 1053